Journal: Journal of the American Society of Echocardiography : official publication of the American Society of Echocardiography

Article Title: Therapeutic Ultrasound Increases Myocardial Blood Flow in Ischemic Myocardium and Cardiac Endothelial Cells: Results of In-vivo and In-Vitro Experiments

doi: 10.1016/j.echo.2019.05.012

Figure Lengend Snippet: MCE images from a control dog. Panels A to C depict LAD perfusion bed size, risk area, and ROI’s placed over the myocardium at baseline MCE image (stage A) from where time versus acoustic intensity plots were derived. Panels D and E depict the actual plots derived from the risk area and border zone during coronary occlusion (stage B) and 45 min after treatment period (stage D). Videos 1 and 2 depict the MCE aligned end-systolic images at different PI’s from where the plots were derived at stages B and D, respectively. See text for details.

Article Snippet: Similarly, US resulted in maintenance of transmural MBF in the border zone compared to control. table ft1 table-wrap mode="anchored" t5 Table 4. caption a7 Variables Stage A Stage B Stage C Stage D Stage E Myocardial Blood Volume (A)-Control 68±26 62±12 50±25 32±21 64±26 Myocardial Blood Volume (A)-US 82±13 61±24 61±15 58±26 64±19 MBF Velocity (β)-Control 0.44±0.19 0.27±0.20 0.22±0.12 0.29±0.19 0.30±0.14 MBF Velocity (β)-US 0.47±0.18 0.28±0.13 0.32±0.15 0.29±0.18 0.32±0.20 Total MBF (A·β)-Control 30±20 16±13 10±8 9±6 18±8 Total MBF (A·β)-US 37±12 17±10 20±10 * 15±8 19±13 Open in a separate window Stage A=baseline Stage B=LAD occlusion prior to treatment Stage C=LAD occlusion with or without treatment Stage D=LAD occlusion 45 min after treatment completion Stage E=90 min after reperfusion US=ultrasound; LAD=left anterior descending artery; MBF=myocardial blood flow; * p=0.03 MCE Results (mean±1SD) from the LAD Border Zone No changes were noted in any of the MCE parameters derived from the LCx bed between US and control groups at or between any of the stages.

Techniques: Derivative Assay

Journal: Journal of the American Society of Echocardiography : official publication of the American Society of Echocardiography

Article Title: Therapeutic Ultrasound Increases Myocardial Blood Flow in Ischemic Myocardium and Cardiac Endothelial Cells: Results of In-vivo and In-Vitro Experiments

doi: 10.1016/j.echo.2019.05.012

Figure Lengend Snippet: MCE images from a dog undergoing US treatment. Panels A to C depict LAD perfusion bed size, risk area, and ROI’s placed over the myocardium at baseline MCE image (stage A) from where time versus acoustic intensity plots were derived. Panels D and E depict the actual plots derived from the risk area and border zone during coronary occlusion (stage B) and 45 min after treatment period (stage D). Videos 3 and 4 depict the MCE aligned end-systolic images at different PI’s from where the plots were derived at stages B and D, respectively. See text for details.

Article Snippet: Similarly, US resulted in maintenance of transmural MBF in the border zone compared to control. table ft1 table-wrap mode="anchored" t5 Table 4. caption a7 Variables Stage A Stage B Stage C Stage D Stage E Myocardial Blood Volume (A)-Control 68±26 62±12 50±25 32±21 64±26 Myocardial Blood Volume (A)-US 82±13 61±24 61±15 58±26 64±19 MBF Velocity (β)-Control 0.44±0.19 0.27±0.20 0.22±0.12 0.29±0.19 0.30±0.14 MBF Velocity (β)-US 0.47±0.18 0.28±0.13 0.32±0.15 0.29±0.18 0.32±0.20 Total MBF (A·β)-Control 30±20 16±13 10±8 9±6 18±8 Total MBF (A·β)-US 37±12 17±10 20±10 * 15±8 19±13 Open in a separate window Stage A=baseline Stage B=LAD occlusion prior to treatment Stage C=LAD occlusion with or without treatment Stage D=LAD occlusion 45 min after treatment completion Stage E=90 min after reperfusion US=ultrasound; LAD=left anterior descending artery; MBF=myocardial blood flow; * p=0.03 MCE Results (mean±1SD) from the LAD Border Zone No changes were noted in any of the MCE parameters derived from the LCx bed between US and control groups at or between any of the stages.

Techniques: Derivative Assay

Journal: Journal of the American Society of Echocardiography : official publication of the American Society of Echocardiography

Article Title: Therapeutic Ultrasound Increases Myocardial Blood Flow in Ischemic Myocardium and Cardiac Endothelial Cells: Results of In-vivo and In-Vitro Experiments

doi: 10.1016/j.echo.2019.05.012

Figure Lengend Snippet: Hemodynamic Results (mean±1SD)

Article Snippet: Similarly, US resulted in maintenance of transmural MBF in the border zone compared to control. table ft1 table-wrap mode="anchored" t5 Table 4. caption a7 Variables Stage A Stage B Stage C Stage D Stage E Myocardial Blood Volume (A)-Control 68±26 62±12 50±25 32±21 64±26 Myocardial Blood Volume (A)-US 82±13 61±24 61±15 58±26 64±19 MBF Velocity (β)-Control 0.44±0.19 0.27±0.20 0.22±0.12 0.29±0.19 0.30±0.14 MBF Velocity (β)-US 0.47±0.18 0.28±0.13 0.32±0.15 0.29±0.18 0.32±0.20 Total MBF (A·β)-Control 30±20 16±13 10±8 9±6 18±8 Total MBF (A·β)-US 37±12 17±10 20±10 * 15±8 19±13 Open in a separate window Stage A=baseline Stage B=LAD occlusion prior to treatment Stage C=LAD occlusion with or without treatment Stage D=LAD occlusion 45 min after treatment completion Stage E=90 min after reperfusion US=ultrasound; LAD=left anterior descending artery; MBF=myocardial blood flow; * p=0.03 MCE Results (mean±1SD) from the LAD Border Zone No changes were noted in any of the MCE parameters derived from the LCx bed between US and control groups at or between any of the stages.

Techniques:

Journal: Journal of the American Society of Echocardiography : official publication of the American Society of Echocardiography

Article Title: Therapeutic Ultrasound Increases Myocardial Blood Flow in Ischemic Myocardium and Cardiac Endothelial Cells: Results of In-vivo and In-Vitro Experiments

doi: 10.1016/j.echo.2019.05.012

Figure Lengend Snippet: MCE Results (mean±1SD) from the LAD Risk Area

Article Snippet: Similarly, US resulted in maintenance of transmural MBF in the border zone compared to control. table ft1 table-wrap mode="anchored" t5 Table 4. caption a7 Variables Stage A Stage B Stage C Stage D Stage E Myocardial Blood Volume (A)-Control 68±26 62±12 50±25 32±21 64±26 Myocardial Blood Volume (A)-US 82±13 61±24 61±15 58±26 64±19 MBF Velocity (β)-Control 0.44±0.19 0.27±0.20 0.22±0.12 0.29±0.19 0.30±0.14 MBF Velocity (β)-US 0.47±0.18 0.28±0.13 0.32±0.15 0.29±0.18 0.32±0.20 Total MBF (A·β)-Control 30±20 16±13 10±8 9±6 18±8 Total MBF (A·β)-US 37±12 17±10 20±10 * 15±8 19±13 Open in a separate window Stage A=baseline Stage B=LAD occlusion prior to treatment Stage C=LAD occlusion with or without treatment Stage D=LAD occlusion 45 min after treatment completion Stage E=90 min after reperfusion US=ultrasound; LAD=left anterior descending artery; MBF=myocardial blood flow; * p=0.03 MCE Results (mean±1SD) from the LAD Border Zone No changes were noted in any of the MCE parameters derived from the LCx bed between US and control groups at or between any of the stages.

Techniques:

Journal: Journal of the American Society of Echocardiography : official publication of the American Society of Echocardiography

Article Title: Therapeutic Ultrasound Increases Myocardial Blood Flow in Ischemic Myocardium and Cardiac Endothelial Cells: Results of In-vivo and In-Vitro Experiments

doi: 10.1016/j.echo.2019.05.012

Figure Lengend Snippet: MCE Results (mean±1SD) from the LAD Border Zone

Article Snippet: Similarly, US resulted in maintenance of transmural MBF in the border zone compared to control. table ft1 table-wrap mode="anchored" t5 Table 4. caption a7 Variables Stage A Stage B Stage C Stage D Stage E Myocardial Blood Volume (A)-Control 68±26 62±12 50±25 32±21 64±26 Myocardial Blood Volume (A)-US 82±13 61±24 61±15 58±26 64±19 MBF Velocity (β)-Control 0.44±0.19 0.27±0.20 0.22±0.12 0.29±0.19 0.30±0.14 MBF Velocity (β)-US 0.47±0.18 0.28±0.13 0.32±0.15 0.29±0.18 0.32±0.20 Total MBF (A·β)-Control 30±20 16±13 10±8 9±6 18±8 Total MBF (A·β)-US 37±12 17±10 20±10 * 15±8 19±13 Open in a separate window Stage A=baseline Stage B=LAD occlusion prior to treatment Stage C=LAD occlusion with or without treatment Stage D=LAD occlusion 45 min after treatment completion Stage E=90 min after reperfusion US=ultrasound; LAD=left anterior descending artery; MBF=myocardial blood flow; * p=0.03 MCE Results (mean±1SD) from the LAD Border Zone No changes were noted in any of the MCE parameters derived from the LCx bed between US and control groups at or between any of the stages.

Techniques:

Journal: Journal of the American Society of Echocardiography : official publication of the American Society of Echocardiography

Article Title: Therapeutic Ultrasound Increases Myocardial Blood Flow in Ischemic Myocardium and Cardiac Endothelial Cells: Results of In-vivo and In-Vitro Experiments

doi: 10.1016/j.echo.2019.05.012

Figure Lengend Snippet: Regional Wall Thickening Results (mean±1SD)

Article Snippet: Similarly, US resulted in maintenance of transmural MBF in the border zone compared to control. table ft1 table-wrap mode="anchored" t5 Table 4. caption a7 Variables Stage A Stage B Stage C Stage D Stage E Myocardial Blood Volume (A)-Control 68±26 62±12 50±25 32±21 64±26 Myocardial Blood Volume (A)-US 82±13 61±24 61±15 58±26 64±19 MBF Velocity (β)-Control 0.44±0.19 0.27±0.20 0.22±0.12 0.29±0.19 0.30±0.14 MBF Velocity (β)-US 0.47±0.18 0.28±0.13 0.32±0.15 0.29±0.18 0.32±0.20 Total MBF (A·β)-Control 30±20 16±13 10±8 9±6 18±8 Total MBF (A·β)-US 37±12 17±10 20±10 * 15±8 19±13 Open in a separate window Stage A=baseline Stage B=LAD occlusion prior to treatment Stage C=LAD occlusion with or without treatment Stage D=LAD occlusion 45 min after treatment completion Stage E=90 min after reperfusion US=ultrasound; LAD=left anterior descending artery; MBF=myocardial blood flow; * p=0.03 MCE Results (mean±1SD) from the LAD Border Zone No changes were noted in any of the MCE parameters derived from the LCx bed between US and control groups at or between any of the stages.

Techniques:

Journal: Nutrients

Article Title: Lipoic Acid Exacerbates Oxidative Stress and Lipid Accumulation in the Liver of Wistar Rats Fed a Hypercaloric Choline-Deficient Diet

doi: 10.3390/nu13061999

Figure Lengend Snippet: Effects of α-lipoic acid (LA) supplementation on body weight and nutritional profile.

Article Snippet: Animals were divided into three groups ( n = 8 in each): control pair-feeding group of rats fed a control diet (C) ( ); rats fed a hypercaloric choline-deficient diet (HCCD); rats fed a hypercaloric choline-deficient diet supplemented with α-lipoic acid (LA) (Chem Impex International, Inc., Wood Dale, USA, Cat# 29862) (HCCD+LA; consumed average daily dose of LA-61 mg/kg body weight).

Techniques:

Journal: Nutrients

Article Title: Lipoic Acid Exacerbates Oxidative Stress and Lipid Accumulation in the Liver of Wistar Rats Fed a Hypercaloric Choline-Deficient Diet

doi: 10.3390/nu13061999

Figure Lengend Snippet: Effects of α-lipoic acid (LA) supplementation on: ( a ) liver/body weight ratio; ( b ) visceral adipose tissue (VAT)/body weight ratio. Animals were divided into three groups: control group of rats fed a control diet (C); rats fed a hypercaloric choline-deficient diet (HCCD); rats fed a hypercaloric choline-deficient diet supplemented with α-lipoic acid (HCCD+LA; consumed average daily dose of LA-61 mg/kg body weight). VAT: visceral (epididymal and retroperitoneal) adipose tissue. Values are mean ± standard error of the mean. p < 0.05 vs. * C.

Article Snippet: Animals were divided into three groups ( n = 8 in each): control pair-feeding group of rats fed a control diet (C) ( ); rats fed a hypercaloric choline-deficient diet (HCCD); rats fed a hypercaloric choline-deficient diet supplemented with α-lipoic acid (LA) (Chem Impex International, Inc., Wood Dale, USA, Cat# 29862) (HCCD+LA; consumed average daily dose of LA-61 mg/kg body weight).

Techniques:

Journal: Nutrients

Article Title: Lipoic Acid Exacerbates Oxidative Stress and Lipid Accumulation in the Liver of Wistar Rats Fed a Hypercaloric Choline-Deficient Diet

doi: 10.3390/nu13061999

Figure Lengend Snippet: Effects of α-lipoic acid (LA) supplementation on liver lipid content.

Article Snippet: Animals were divided into three groups ( n = 8 in each): control pair-feeding group of rats fed a control diet (C) ( ); rats fed a hypercaloric choline-deficient diet (HCCD); rats fed a hypercaloric choline-deficient diet supplemented with α-lipoic acid (LA) (Chem Impex International, Inc., Wood Dale, USA, Cat# 29862) (HCCD+LA; consumed average daily dose of LA-61 mg/kg body weight).

Techniques:

Journal: Nutrients

Article Title: Lipoic Acid Exacerbates Oxidative Stress and Lipid Accumulation in the Liver of Wistar Rats Fed a Hypercaloric Choline-Deficient Diet

doi: 10.3390/nu13061999

Figure Lengend Snippet: Effects of α-lipoic acid (LA) supplementation on plasma levels of ( a ) glucose, ( b ) triglycerides (TG), ( c ) free fatty acids (FFA), ( d ) total cholesterol (T-Chol), ( e ) high-density lipoprotein cholesterol (HDL-Chol), ( f ) low-density lipoprotein cholesterol (LDL-Chol). Animals were divided into three groups: control group of rats fed a control diet (C); rats fed a hypercaloric choline-deficient diet (HCCD); rats fed a hypercaloric choline-deficient diet supplemented with α-lipoic acid (HCCD+LA; consumed average daily dose of LA-61 mg/kg body weight). Values are mean ± standard error of the mean. p < 0.05 vs. * C; # HCCD+LA vs. HCCD.

Article Snippet: Animals were divided into three groups ( n = 8 in each): control pair-feeding group of rats fed a control diet (C) ( ); rats fed a hypercaloric choline-deficient diet (HCCD); rats fed a hypercaloric choline-deficient diet supplemented with α-lipoic acid (LA) (Chem Impex International, Inc., Wood Dale, USA, Cat# 29862) (HCCD+LA; consumed average daily dose of LA-61 mg/kg body weight).

Techniques:

Journal: Nutrients

Article Title: Lipoic Acid Exacerbates Oxidative Stress and Lipid Accumulation in the Liver of Wistar Rats Fed a Hypercaloric Choline-Deficient Diet

doi: 10.3390/nu13061999

Figure Lengend Snippet: Effects of α-lipoic acid (LA) supplementation on ( a ) alanine aminotransferase (ALT) activity in plasma; ( b ) aspartate aminotransferase (AST) activity in plasma; ( c ) reduced glutathione (GSH) level in the liver; ( d ) reduced/oxidized glutathione ratio (GSH/GSSG) in the liver; ( e ) total antioxidant (AOA) activity in plasma; ( f ) total antioxidant (AOA) activity in the liver; ( g ) cytochrome P450 2E1 (CYP2E1) activity in the liver. Animals were divided into three groups: control group of rats fed a control diet (C); rats fed a hypercaloric choline-deficient diet (HCCD); rats fed a hypercaloric choline-deficient diet supplemented with α-lipoic acid (HCCD+LA; consumed average daily dose of LA-61 mg/kg body weight). Values are mean ± standard error of the mean. p < 0.05 vs. * C; # HCCD+LA vs. HCCD.

Article Snippet: Animals were divided into three groups ( n = 8 in each): control pair-feeding group of rats fed a control diet (C) ( ); rats fed a hypercaloric choline-deficient diet (HCCD); rats fed a hypercaloric choline-deficient diet supplemented with α-lipoic acid (LA) (Chem Impex International, Inc., Wood Dale, USA, Cat# 29862) (HCCD+LA; consumed average daily dose of LA-61 mg/kg body weight).

Techniques: Activity Assay

Journal: Nutrients

Article Title: Lipoic Acid Exacerbates Oxidative Stress and Lipid Accumulation in the Liver of Wistar Rats Fed a Hypercaloric Choline-Deficient Diet

doi: 10.3390/nu13061999

Figure Lengend Snippet: Effects of α-lipoic acid (LA) supplementation on ( a ) catalase (CAT) activity in the liver; ( b ) glutathione peroxidase (GPx) activity in the liver; ( c ) superoxide dismutase (SOD) activity in the liver; ( d ) paraoxonase-1 (PON-1) activity in plasma; ( e ) paraoxonase-1 (PON-1) activity in the liver. Animals were divided into three groups: control group of rats fed a control diet (C); rats fed a hypercaloric choline-deficient diet (HCCD); rats fed a hypercaloric choline-deficient diet supplemented with α-lipoic acid (HCCD+LA; consumed average daily dose of LA-61 mg/kg body weight). Values are mean ± standard error of the mean. p < 0.05 vs. * C; # HCCD+LA vs. HCCD.

Article Snippet: Animals were divided into three groups ( n = 8 in each): control pair-feeding group of rats fed a control diet (C) ( ); rats fed a hypercaloric choline-deficient diet (HCCD); rats fed a hypercaloric choline-deficient diet supplemented with α-lipoic acid (LA) (Chem Impex International, Inc., Wood Dale, USA, Cat# 29862) (HCCD+LA; consumed average daily dose of LA-61 mg/kg body weight).

Techniques: Activity Assay

Journal: Nutrients

Article Title: Lipoic Acid Exacerbates Oxidative Stress and Lipid Accumulation in the Liver of Wistar Rats Fed a Hypercaloric Choline-Deficient Diet

doi: 10.3390/nu13061999

Figure Lengend Snippet: Effects of α-lipoic acid (LA) supplementation on histological features of the liver (portal area, ×100): ( a ) control group, hematoxylin-eosin stain; ( b ) control group, van Gieson stain; ( c ) hypercaloric choline-deficient diet group, hematoxylin-eosin stain; ( d ) hypercaloric choline-deficient diet group, van Gieson stain; ( e ) hypercaloric choline-deficient diet supplemented with α-lipoic acid (consumed average daily dose of LA-61 mg/kg body weight), hematoxylin-eosin stain; ( f ) hypercaloric choline-deficient diet supplemented with α-lipoic acid (consumed average daily dose of LA-61 mg/kg body weight), van Gieson stain. Large-sized lipid droplets are indicated by the blue arrow, foci of lobular inflammation by the black arrow, and hepatocellular ballooning by the red arrow. Scale bar = 500 μm.

Article Snippet: Animals were divided into three groups ( n = 8 in each): control pair-feeding group of rats fed a control diet (C) ( ); rats fed a hypercaloric choline-deficient diet (HCCD); rats fed a hypercaloric choline-deficient diet supplemented with α-lipoic acid (LA) (Chem Impex International, Inc., Wood Dale, USA, Cat# 29862) (HCCD+LA; consumed average daily dose of LA-61 mg/kg body weight).

Techniques: Staining